Mebiol
Hydrogels are a diverse class of polymer materials that are characterized by their network-like structure and high water content. Hydrogels of many types find a wide range of applications in medicine and life science research, but are not limited to three-dimensional cell culture, tissue engineering and drug delivery. Properties that are very beneficial for cell culture and tissue engineering prompted the commercialization of Mebiol Gel, a copolymer of poly (N-isopropylacrylamide) and poly (ethylene glycol) (PNIPAAm-PEG) for research purposes in the early 2000s. In contrast to other commercially available hydrogels, the defining characteristic of Mebiol Gel is the temperature-reversible sol-gel transition. When cooled, Mebiol Gel is soluble (treated like a liquid), but becomes a rigid hydrogel at higher temperatures. In practice, this means that the cells are very easy to handle. The cultures are sown in chilled Mebiol gel and conveniently obtained by cooling the culture vessel and centrifuging. In the gel state, the highly lipophilic environment of the Mebiol Gel presents an efficient niche for cell proliferation, cell communication, gas and mass exchange and protection of cells and tissues from shear forces.
Low temperature 4 ° C (soluble) High temperature 37 ° C (gel)
Applications
- Stem cell and pluripotent stem cell culture
- Expansion and differentiation of spheroid cultures
- Cell implantation
- Organ and tissue regeneration
- Drug delivery
- Non-cell culture applications
properties
- Easy to use
- Non-toxic, biocompatible
- 100% synthetic,
- pathogen free
- High transparency for cell observation
- Proven performance
Products
| product name | Catalog no. | Quantity |
| Mebiol gel | MBG-PMW20-1001 | 1 * 10ML |
| Mebiol gel | MBG-PMW20-5001 | 1 * 50ML |
| Mebiol gel | MBG-PMW20-1005 | 5 * 10ML |
| Mebiol gel | MBG-PMW20-5005 | 5 * 50ML |
credentials
Cell culture and tissue regeneration
| Stem Cells (human pluipotent stem cell (hPSC) line expansion and differentiation) |
| Defined and Scalable Differentiation of Human Oligodendrocyte Precursors from Pluripotent Stem Cells in a 3D Culture System. Rodrigues GMC, Gaj T, Adil MM, Wahba J, Rao AT, Lorbeer FK, Kulkarni RU, Diogo MM, Cabral JMS, Miller EW, Hockemeyer D, Schaffer DV. Stem Cell Reports. 2017 Jun 6; 8 (6): 1770-1783. PMID: 28552605 |
| Review |
| Engineering-derived approaches for iPSC preparation, expansion, differentiation and applications. Li Y, Li L, Chen ZN, Gao G, Yao R, Sun W. Biofabrication. 2017 Jul 31; 9 (3): 032001. PMID: 28759433 |
| Stem Cells (human pluipotent stem cell (hPSC) line expansion and differentiation) |
| An Integrated Miniature Bioprocessing for Personalized Human Induced Pluripotent Stem Cell Expansion and Differentiation into Neural Stem Cells. Haishuang Lin, Qiang Li, Yuguo Lei Sci Rep. 2017 Jan 6; 7: 40191. doi: 10.1038 / srep40191. PMID: 28057917 |
| Adoptive transfer of T cells into recipient mice |
| Obesity accelerates T cell senescence in murine visceral adipose tissue. Shirakawa K, Yan X, Shinmura K, Endo J, Kataoka M, Katsumata Y, Yamamoto T, Anzai A, Isobe S, Yoshida N, Itoh H, Manabe I, Sekai M, Hamazaki Y, Fukuda K, Minato N, Sano M . J Clin Invest. 2016 Dec 1; 126 (12): 4626-4639. PMID: 27820698 |
| Review |
| Stem Cell Organoid Engineering Xiaolei Yin, Benjamin E. Mead, Helia Safaee, Robert Langer, Jeffrey M. Karp and Oren Levy Cell Stem Cell. 2016 Jan 7; 18 (1): 25-38. PMID: 26748754 |
| Affordable glioblastoma TICs for drug discovery |
| Scalable Production of Glioblastoma Tumor-initiating Cells in 3 Dimension Thermoreversible Hydrogels. Qiang Li, Haishuang Lin, Ou Wang, Xuefeng Qiu, Srivatsan Kidambi, Loic P. Deleyrolle, Brent A. Reynolds & Yuguo Lei. Scientific Reports 6, Article number: 31915 (2016) PMID: 27549983 |
| Biological processes |
| Light-Patterned RNA Interference of 3D-Cultured Human Embryonic Stem Cells. Xiao Huang, Qirui Hu, Yifan Lai, Demosthenes P. Morales, Dennis O. Clegg and Norbert O. Reich DOI: 10.1002 / adma.201603318 PMID: 27787919 |
| Osteopontin Production |
| A CD153 + CD4 + T Follicular Cell Population with Cell-Senescence Features Plays a Crucial Role in Lupus Pathogenesis via Osteopontin Production Suhail Tahir, Yuji Fukushima, Keiko Sakamoto, Kyosuke Sato, Harumi Fujita, Joe Inoue, Toshimitsu Uede, Yoko Hamazaki, Masakazu Hatt and Nagahiro Minato J. Immunol., Jun 2015; 194: 5725-5735. PMID: 25972477 |
| Stem Cells (human pluipotent stem cell (hPSC) line expansion and differentiation) |
| Developing Defined and Scalable 3D Culture Systems for Culturing Human Pluripotent Stem Cells at High Densities. Yuguo Lei, Daeun Jeong, Jifang Xiao, David V. Schaffer Cell Mol Bioeng. 2014 Jun; 7 (2): 172-183. PMID: 25419247 |
| Review |
| Scalable culture of human pluripotent stemcells in 3D Todd C. McDevitt Proc Natl Acad Sci US A. 2013 Dec 24; 110 (52): 20852-3. PMID: 24327732 |
| Stem Cells (corneal limbal), review |
| Towards the use of hydrogels in the treatment of limbal stem cell deficiency. Bernice Wright, Shengli Mi, and Che J. Connon Drug Discov Today. 2013 Jan; 18 (1-2): 79-86. PMID: 22846850 |
| Stem Cells (human pluipotent stem cell (hPSC) line expansion and differentiation) |
| A fully defined and scalable 3D culture system for human pluripotent stem cell expansion and differentiation. Yuguo Leia, David V. Schaffer Proc Natl Acad Sci US A. 2013 Dec 24; 110 (52): E5039-48. PMID: 24248365 |
| Cell Culture (kidney cyst formation) |
| Mxi1 influences cyst formation in three-dimensional cell culture. Yook YJ, Yoo KH, Song SA, Seo MJ, Ko JY, Kim BH, Lee EJ, Chang E, Woo YM, Park JH BMB Rep. 2012 Mar; 45 (3): 189-93. PMID: 22449707 |
| ROS cell-based assay |
| Effect of green tea extract on reactive oxygen species produced by neutrophils from cancer patients. Katsuhiko Suzuki, Satoshi Ohno, Yoko Suzuki, Yumiko Ohno, Ryuji Okuyama, Atsushi Aruga, Masakazu Yamamoto, Ken-O Ishihara, Tsutomu Nozaki, Shigeki Miura, Hiroshi Yoshioka, Yuichi Mori Anticancer Res. 2012 Jun; 32: 32 (32) 75. PMID: 22641677 |
| ROS cell-based assay |
| Determination of chronic inflammatory states in cancer patients using assay of reactive oxygen species production by neutrophils. Suzuki Y, Ohno S, Okuyama R, Aruga A, Yamamoto M, Miura S, Yoshioka H, Mori Y, Suzuki K Anticancer Res. 2012 Feb; 32 (2): 565-70 PMID: 22287746 |
| Cell Culture for drug screening |
| In vitro Thermoreversible Gel Disc Quantitative Assay of Rat Angiogenesis. Shin Wakui, Tomoko Muto, Naohiko Anzai, Hiroyuki Takahashi, Promsuk Jutabha, Akihiko Sakata, Michael F. Wempe, Hiroshi Hano, Hitoshi Endou AATEX 16 (2), 59-65, 2011 PMID: |
| Wound healing |
| Wound dressing of newly developed thermobelling thermoreversible hydrogel. H. Yoshioka, Y. Mori, S. Kubota Jinko Zoki Vol. 27 (1998) No. 2 P 503-506 PMID: |
| Organ Culture |
| FGF signaling directs a center-to-pole expansion of tubulogenesis in mouse testis differentiation. Hiramatsu R, Harikae K, Tsunekawa N, Kurohmaru M, Matsuo I, Kanai Y Development. 2010 Jan; 137 (2): 303-12. PMID: 20040496 |
| Stem Cell Culture, Regenerative Medicine |
| Application of a Thermo-Reversible Gelation Polymer, Mebiol Gel, for Stem Cell Culture and Regenerative Medicine. Kataoka K, Huh N Journal of Stem Cell & Regenerative Medicine 2010 Vol. 6 (1): p10-14 (2010) PMID: 24693055 |
| Embryo Explant Culture |
| Antagonism between Smad1 and Smad2 signaling determines the site of distal visceral endoderm formation in the mouse embryo. Yamamoto M, Beppu H, Takaoka K, Meno C, Li E, Miyazono K, Hamada H J Cell Biol. 2009 Jan 26; 184 (2): 323-34 PMID: 19153222 |
| Virus infection / replication system |
| 3D cultured immortalized human hepatocytes useful to develop drugs for blood-borne HCV. Aly HH, Shimotohno K, Hijikata M Biochem Biophys Res Commun. 2009 Feb 6; 379 (2): 330-4. PMID: 19103167 |
| Stem cells (corneal limbal) |
| Limbal stem cells: application in ocular biomedicine. Vemuganti GK, Fatima A, Madhira SL, Basti S, Sangwan VS Int Rev Cell Mol Biol. 2009; 275: 133-81. PMID: 19491055 |
| Stem cells (corneal limbal) |
| Ex vivo cultivation of corneal limbal epithelial cells in a thermoreversible polymer (Mebiol Gel) and their transplantation in rabbits: an animal model. Sitalakshmi G, Sudha B, Madhavan HN, Vinay S, Krishnakumar S, Mori Y, Yoshioka H, Abraham S. Tissue Eng Part A. 2009 Feb; 15 (2): 407-15. PMID: 18724830 |
| Stem Cells (mesenchymal) |
| Chondrogenic differentiation of human mesenchymal stem cells from umbilical cord blood in chemically synthesized thermoreversible polymer. I. Kao, C. Yao, Y. Chang, T. Hsieh, S. Hwang, Chin J Physiol. 2008 Aug 31; 51 (4): 252-8 PMID: 19112883 |
| Hepatocyte transplant |
| Intraperitoneal Transplantation of Hepatocytes Embedded in Thermoreversible Gelation Polymer (Mebiol Gel) in Acute Liver Failure Rat Model. N. Parveen, AA Khan, S. Baskar, MA Habeeb, P. Ravindra Babu, A. Samuel, Y. Hiroshi, M. Yuichi, CM Habibullah Hepatitis Monthly, Volume 275 8, Issue 4, Autumn, November 2008 Page S71 PMID : |
| Stem Cells (Corneal Limbal) |
| A Rabbit Model of Ex Vivo Cultivation and Transplantation of Autologous Limbal Epithelial Cells Grown in a Thermo-reversible Gelation Polymer (Mebiol Gel). Sitalakshmi G, Sudha B, Vinay S, Madhavan H, Krishnakumar S, Mori Y, Yoshioka H, Abraham S J Stem Cells Regen Med. 2006 Dec 26; 1 (1): 44-5. eCollection 2006. PMID: 24692863 |
| Virus Reproduction and Drug Screening |
| Production of infectious hepatitis C virus particles in three-dimensional cultures of the cell line carrying the genome-length dicistronic viral RNA of genotype 1b. K. Murakami, K. Ishii, Y. Ishihara, S. Yoshizaki, K. Tanaka. Y. Gotoh, H. Aizaki, M. Kohara, H. Yoshioka, Y. Mori, N. Manabe, I., Shoji, T. Sata, R. Bartenschlarger, Y. Matsuura, T. Miyamura, T. Suzuki, Virology . 2006 Aug 1; 351 (2): 381-92. Epub 2006 May 6 PMID: 16678876 |
| Tissue Regeneration (Liver) |
| Thermoreversible gelation polymer induces the emergence of hepatic stem cells in the partially injured rat liver. Nagaya, M., Kubota, S., Suzuki, N., Akashi, K., Mitaka, T., Hepatology. 2006 May; 43 (5): 1053-62. PMID: 16628635 |
| Stem Cells (epithelial) |
| Isolation of epithelial stem cells from dermis by a three-dimensional culture system. Reinhold J. Medina, Ken Kataoka, Mikiro Takaishi, Masahiro Miyazaki, Nam-ho Huh, J Cell Biochem. 2006 May 1; 98 (1): 174-84. PMID: 16408300 |
| Tissue engineering (bone) |
| In Vitro Culture of Chondrocytes in a Novel Thermoreversible Gelation Polymer Scaffold Containing Growth Factors. Yasuda, K. Kojima, KW Tinsley, H. Yoshioka, Y. Mori, CA Vacanti, Tissue Eng. 2006 May; 12 (5): 1237-45. PMID: 16771637 |
| Stem cells (corneal limbal) |
| Cultivation of human corneal limbal stem cells in Mebiol Gel A thermoreversible gelation polymer. B. Sudha, HN Madhavan, G. Sitalakshmi, J. Malathi, S. Krishnakumar, Y. Mori, H. Yoshioka, S. Abraham, Indian J Med Res. 2006 Dec; 124 (6): 655-64. PMID: 17287553 |
| Hepatocyte culture |
| Serum-derived hepatitis C virus infectivity in interferon regulatory factor-7-suppressed human primary hepatocytes. Aly HH, Watashi K, Hijikata M, Kaneko H, Takada Y, Egawa H, Uemoto S, Shimotohno K J Hepatol. 2007 Jan; 46 (1): 26-36. Epub 2006 Oct 30th PMID: 17112629 |
| Embryo culture |
| Canonical Wnt Signaling and Its Antagonist Regulate Anterior-Posterior Axis Polarization by Guiding Cell Migration in Mouse Visceral Endoderm. Kimura-Yoshida C, Nakano H, Okamura D, Nakao K, Yonemura S, Belo JA, Aizawa S, Matsui Y, Matsuo I. Dev Cell. 2005 Nov; 9 (5): 639-50. PMID: 16256739 |
| Liver regeneration |
| Evaluation of thermoreversible gelation polymer for regeneration of focal liver injury. M. Nagaya, S. Kubota, N. Suzuki, M. Tadokoro, K. Akashi, Eur Surg Res. 2004 Mar-Apr; 36 (2): 95-103. PMID: 15007262 |
| Stem Cell Culture and Differentiation |
| Gene expression profile of human mesenchymal stem cells during osteogenesis in three-dimensional thermoreversible gelation polymer. Hishikawa, K., Miura, S., Marumo, T., Yoshioka, H., Mori, Y., Takato, T., Fujita, T., Biochem Biophys Res Commun. 2004 May 14; 317 (4): 1103-7. PMID: 15094382 |
| Evalulation of Cell Line Growth in Mebiol Gel |
| A study on the growth of continuous culture cell lines embedded in Mebiol Gel. HN Madhavan, J. Malathi, Patricia, Rinku Joseph, Yuichi Mori, Samuel JK Abraham and Hiroshi Yoshioka, CURRENT SCIENCE 87 (9), 1275-1277 (2004) PMID: |
| Spheroid Culture (Cancer) |
| A new method to prepare multicellular spheroids in cancer cell lines using a thermo-reversible gelation polymer. Tsukikawa, S., Matsuoka, H., Kurahashi, Y., Konno, Y., Satoh, K., Satoh, R., Isogai, A., Kimura, K., Watanabe, Y., Nakano, S., Hayashi, J., Kubota, S., Artif Organs. 2003 Jul; 27 (7): 598-604. PMID: 12823414 |
| Islet transplant |
| In vitro studies on a new method for islet microencapsulation using a thermoreversible gelation polymer, N-isopropylacrylamide-based copolymer. Shimizu, S., Yamazaki, M., Kubota, S., T. Ozasa, H. Moriya, Kobayashi, K., Mikami, M., Mori, Y., and Yamaguchi, S., Artif Organs. 1996 Nov; 20 (11): 1232-7. PMID: 8908335 |
Non-cell culture applications
| Protein crystallization scaffold |
| Viscous hydrophilic injection matrices for serial crystallography Gabriela Kovacsova, Marie Luise Grunbein, Marco Kloos, Thomas RM Barends, Ramona Schlesinger, Joachim Heberle, Wolfgang Kabsch, Robert L. Shoeman, R. Bruce Doak and Ilme Schlichting IUCrJ Volume 4, Part 4, July 2017, Pages 400-410 PMID: |
| Protein crystallization scaffold |
| A Novel Approach for Protein Crystallization by a Synthetic Hydrogel with Thermoreversible Gelation Polymer. Shigeru Sugiyama, Noriko Shimizu, Gen Sazaki, Mika Hirose, Yoshinori Takahashi, Mihoko Maruyama, Hiroyoshi Matsumura, Hiroaki Adachi, Kazufumi Takano, Satoshi Murakami, Tsuyoshi Inoue, and Yusuke Mori Cryst. Growth Des., 2013, 13 (5), pp 1899-1904 PMID: |
| DNA molecular sorting |
| Microfluidic active sorting of DNA molecules labeled with single quantum dots using flow switching by a hydrogel sol? Gel transition. Mai Haneoka, Yoshitaka Shirasaki, Hirokazu Sugino, Tokihiko Aoki, Takahiro Arakawa, Kazuto Ozaki, Dong Hyun Yoon, Noriyuki Ishii, Ryo Iizuka, Shuichi Shoji, Takashi Funatsu Chemical, Volume 159, Issue 1, November 28, 2011, Pages 314-320 PMID : |
| Drug delivery |
| Novel local drug delivery system using thermoreversible gel in combination with polymeric microspheres or liposomes. Arai T, Benny O, Joki T, Menon LG, Machluf M, Abe T, Carroll RS, Black PM. Anticancer Res. 2010 Apr; 30 (4): 1057-64. PMID: 20530409 |
| Cell sorting switch |
| Microfluidic cell sorter with flow switching triggered by a sol? Gel transition of a thermo-reversible gelation polymer. Kazuto Ozaki, Hirokazu Sugino, Yoshitaka Shirasaki, Tokihiko Aoki, Takahiro Arakawa, Takashi Funatsu, Shuichi Shoji Chemical, Volume 150, Issue 1, September 21, 2010, Pages 449-455 PMID: |
| Cell sorting switch |
| On-Chip Cell Sorting System Using Thermoreversible Gelation Polymer. Yoshitaka Shirasaki, Hirokazu Sugino, Masayasu Tatsuoka, Jun Mizuno, Shuichi Shoji, and Takashi Funatsu IEEE JOURNAL OF SELECTED TOPICS IN QUANTUM ELECTRONICS, VOL. 13, NO. 2, MARCH / APRIL 2007 PMID: |
| Drug delivery |
| Novel drug delivery system using thermoreversible gelation polymer for malignant glioma. T. Arai, T. Joki, M. Akiyama, M. Agawa, Y. Mori, H. Yoshioka, T. Abe, J Neurooncol. 2006 Mar; 77 (1): 9-15. PMID: 16292493 |
| Cell sorting switch |
| On-Chip Cell Sorting System Using Laser-Induced Heating of a Thermoreversible Gelation Polymer to Control Flow. Y. Shirasaki, J. Tanaka, H. Makazu, K. Tashiro, S. Shoji, S. Tsukita, T. Funatsu, Anal Chem. 2006 Feb 1; 78 (3): 695-701. PMID: 16448041 |
| Scaffold for DNA electrophoresis and recovery |
| Separation and recovery of DNA fragments by electrophoresis through a thermoreversible hydrogel composed of poly (ethylene oxide) and poly (propylene oxide). Yoshioka, H., Mori, Y. and Shimizu, M., Anal Biochem. 2003 Dec 15; 323 (2): 218-23. PMID: 14656528 |
Physical properties
| Bio rapid prototyping by extruding / aspirating / refilling thermoreversible hydrogel. Iwami K, Noda T, Ishida K, Morishima K, Nakamura M, Umeda N. Biofabrication PMID: 20811123 |
| Endovascular treatment of experimental aneurysms using a combination of thermoreversible gelation polymer and protection devices: feasibility study. H. Takao, Y. Murayama, T. Saguchi, T. Ishibashi, M. Ebara, K. Irie, H. Yoshioka, Y. Mori, S. Ohtsubo, F. Vinnuela, T. Abe Neurosurgery. 2009 Sep; 65 (3): 601-9; discussion 609. PMID: 19687707 |
| Endovascular treatment of experimental cerebral aneurysms using thermoreversible liquid embolic agents. Takao H, Murayama Y, Saguchi T, Ishibashi T, Ebara M, Irie K, Yoshioka H, Mori Y, Ohtsubo S, Vinuela F, Abe T. Interv Neuroradiol. 2006 Jan 20 PMID: 20569622 |
| Thermoreversible gelation on heating and on cooling of an aqueous gelatin-poly (N-isopropylacrylamide) conjugate. Yoshioka, H., Mori, Y., Tsukikawa, S. and Kubota, S., Polym. Adv. Tech., 9, 155-158 (1998) PMID: |
| A synthetic hydrogel with thermoreversible gelation. III. : an NMR study of the sol-gel transition. Yoshioka, H., Cushman, JA, Mori, Y. and Tsuchida, E., Polym. Adv. Tech., 5, 122-127 (1994) PMID: |
| A synthetic hydrogel with thermoreversible gelation. II.: Effect of added salts. Yoshioka, H., Mikami, M., Mori, Y. and Tsuchida, E., J. Macromol. Sci., A31 (1), 121-125 (1994) PMID: |
| A synthetic hydrogel with thermoreversible gelation. I.: Preparation and rheological properties. Yoshioka, H., Mikami, M., Mori, Y. and Tsuchida, E., J. Macromol. Sci., A31 (1), 113-120 (1994) PMID: |